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Application Review Date
The First Review Date for this job is: September 2, 2020
The teaching and research activities of the Department of Molecular and Cell Biology (MCB) concern the molecular structures and processes of cellular life and their roles in the function, reproduction, and development of living organisms. This agenda covers a broad range of specialized disciplines, including biochemistry, biophysics, molecular biology, structural biology, genetics, genomics, bioinformatics, cell biology, developmental biology, tumor biology, microbiology, immunology, pathogenesis and neurobiology. The types of living organisms from which the departmental faculty draws its working materials are as diverse as its disciplinary specializations, ranging from viruses and microbes through plants, roundworms, annelids, arthropods, and mollusks to fish, amphibia, and mammals. The faculty of the department is organized into five divisions: Biochemistry and Molecular Biology; Cell and Developmental Biology; Genetics, Genomics and Development; Neurobiology; and Immunology and Pathogenesis.
Isolation and generation of mouse embryonic fibroblasts for the lab. This includes weekly thawing, passaging, counting and plating of cells. In addition, this involved to newly isolate MEFs and replenish frozen MEFs stocks. To do this the SRA will learn how to set up breeding's, weaning's, and euthanizing mice.
Assisting other members in the lab with research projects. Specifically, optimizing and testing tissue culture and molecular biology protocols such as differentiation or staining protocols. This will be done with the guidance of the PI and other lab members. This involves planning, designing the experiments discussing the outline of the experiment/ protocol with the PI, conducting the experiments, analyzing results and interpreting data under the supervision of the PI.
Standard molecular biology and tissue culture techniques. The SRA will assist lab members with the maintenance and characterization of human stem cell cultures and genotyping of stem cells. To do this the SRA will learn to isolate DNA from human stem cells, amplify DNA by PCR and prepare samples for sequence analysis. This genotyping will require daily record keeping in laboratory notebook to record the samples analyzed and the attendance at weekly lab meetings to understand the basic objectives of the research project.
Lab support duties including autoclaving waste and ordering and restocking supplies.
Assisting with protocol renewals and safety inspections.
Experience with sterile tissue culture techniques, PCR, basic lab maintenance duties, excellent organizational skills and attention to detail.
Bachelor's degree in the life sciences, preferably Biology, Molecular and Cell Biology, Biochemistry and/or equivalent experience/training.
1-year (cumulative) experience working in a biology research lab.
Experience with sterile tissue culture and molecular biology techniques.
Mouse handling and experience with molecular biology techniques, knowledge of lab safety procedures, capability to design and conduct experiments and analyze data.
Salary & Benefits
This is an exempt, monthly paid position. Annual salary is commensurate with experience.
For information on the comprehensive benefits package offered by the University visit:
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The University of California was chartered in 1868 and its flagship campus - envisioned as a "City of Learning" - was established at Berkeley, on San Francisco Bay. Today the world's premier public university and a wellspring of innovation, UC Berkeley occupies a 1,232 acre campus with a sylvan 178-acre central core. From this home its academic community makes key contributions to the economic and social well-being of the Bay Area, California, and the nation.